电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Recombinant RFP 抗体 (Atto 488)

RFP 适用: Discosoma IF, ICC, FACS 宿主: Alpaca Monoclonal 2B12-2A1 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
产品编号 ABIN4368335
发货至: 中国
  • 抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    抗体类型
    Recombinant Antibody
    片段
    single-domain Antibody (sdAb)
    适用
    • 51
    • 1
    Discosoma
    宿主
    • 23
    • 14
    • 8
    • 5
    • 3
    Alpaca
    Expression System
    E.coli
    克隆类型
    • 30
    • 23
    单克隆
    标记
    • 20
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is conjugated to Atto 488
    应用范围
    • 28
    • 21
    • 15
    • 11
    • 9
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    原理
    Camelid sdAb anti-RFP conjugated with Atto488, Clones 2B12/2A1
    特异性
    Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
    交叉反应 (详细)
    Does not cross-react with GFP or mTagBFP derivatives.
    产品特性
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    纯化方法
    Produced in: E.coli
    免疫原
    RFP
    克隆位点
    2B12-2A1
    Labeling Ratio
    A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
    Featured
    Discover our best selling RFP Primary Antibody
    Top Product
    Discover our top product RFP Primary Antibody
  • 应用备注
    Recommended dilution 1:500
    说明

    A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    5 μM
    缓冲液
    1.25 μM of each fluorescently labeled sdAb clone in buffered saline, 50 % glycerol, 0.09 % sodium azide. 5 μM total fluorophore concentration
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light!
    储存条件
    -20 °C,-80 °C
    储存方法
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    有效期
    12 months
  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    别名
    RFP (RFP 产品)
    分子量
    26 kDa
You are here:
客服