Recombinant RFP 抗体 (Atto 488)
Quick Overview for Recombinant RFP 抗体 (Atto 488) (ABIN4368335)
抗原
See all RFP 抗体抗体类型
片段
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Expression System
- E.coli
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原理
- Camelid sdAb anti-RFP conjugated with Atto488, Clones 2B12/2A1
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特异性
- Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
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交叉反应 （详细）
- Does not cross-react with GFP or mTagBFP derivatives.
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产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
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纯化方法
- Produced in: E.coli
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免疫原
- RFP
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Labeling Ratio
- A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
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anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA, IP, FACS, FM 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF, FM 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) (N-Term) antibodyRFP 适用: Discosoma WB, ELISA, IF, IHC, IP, DB 宿主: 小鼠 Monoclonal RF5R unconjugated
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应用备注
- Recommended dilution 1:500
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说明
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A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.
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浓度
- 5 μM
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缓冲液
- lyophilized from PBS pH7.4 with 2% BSA (US-Origin)
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注意事项
- Protect from light!
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储存条件
- -20 °C,-80 °C
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储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
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有效期
- 12 months
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- RFP (Red Fluorescent Protein (RFP))
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别名
- RFP
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分子量
- 26 kDa
抗原
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