Recombinant RFP 抗体 (AZDye 568)
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- 抗原 See all RFP 抗体
- RFP (Red Fluorescent Protein (RFP))
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
- 适用
- Discosoma
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宿主
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Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- This RFP antibody is conjugated to AZDye 568
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应用范围
- Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 2B12
- 特异性
- Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
- 交叉反应 （详细）
- Does not cross-react with GFP or mTagBFP derivatives.
- 产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- 纯化方法
- Produced in: E.coli
- 免疫原
- Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)
- 克隆位点
- 2B12
- Labeling Ratio
- a single fluorophore is coupled to exactly one sdAb
- Featured
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anti-Red Fluorescent Protein (RFP) antibody
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anti-Red Fluorescent Protein (RFP) antibodyRFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (Biotin)RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin
anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633
anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488
anti-Red Fluorescent Protein (RFP) antibody (HRP)RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal HRP
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- 应用备注
- Recommended dilution 1:500
- 说明
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Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Add 200 µl H2O for reconstitution. Possibly add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! - 浓度
- 5 μM
- 缓冲液
- 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- 有效期
- 12 months
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- 抗原
- RFP (Red Fluorescent Protein (RFP))
- 别名
- RFP (RFP 产品)
- 分子量
- 26 kDa
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