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Recombinant RFP 抗体 (Atto 488)

RFP 适用: Discosoma IF, ICC, FACS 宿主: Alpaca Monoclonal 2B12 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
产品编号 ABIN4368319
发货至: 中国
  • 抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    抗体类型
    Recombinant Antibody
    片段
    single-domain Antibody (sdAb)
    适用
    • 51
    • 1
    Discosoma
    宿主
    • 23
    • 14
    • 8
    • 5
    • 3
    Alpaca
    Expression System
    E.coli
    克隆类型
    • 30
    • 23
    单克隆
    标记
    • 20
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is conjugated to Atto 488
    应用范围
    • 28
    • 21
    • 15
    • 11
    • 9
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    原理
    Camelid sdAb anti-RFP conjugated with Atto488, Clone 2B12
    特异性
    Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
    交叉反应 (详细)
    Does not cross-react with GFP or mTagBFP derivatives.
    产品特性
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    纯化方法
    Produced in: E.coli
    免疫原
    RFP
    克隆位点
    2B12
    Labeling Ratio
    a single fluorophore is coupled to exactly one sdAb
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  • 应用备注
    Recommended dilution 1:500
    说明

    Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    5 μM
    缓冲液
    5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light!
    储存条件
    -20 °C,-80 °C
    储存方法
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    有效期
    12 months
  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    别名
    RFP (RFP 产品)
    分子量
    26 kDa
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