GFP 抗体 (Texas Red (TR))
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北京 101111
Quick Overview for GFP 抗体 (Texas Red (TR)) (ABIN401757)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts to Green Fluorescent Protein (GFP).
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产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 3.0 moles Texas Red(TM) per mole of Mouse IgG -
纯化方法
- Affinity chromatography on Protein A
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免疫原
- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
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亚型
- IgG2a
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), EM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 1-100) antibodyGFP 适用: Aequorea victoria WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA. Western Blot. Immunohistochemistry.
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限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm where ε0.1% = 0.71)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution stable for a short term at 2-8 °C, but best stored at -20 °C. Following reconstitution store the antibody at -20 °C. Do not aliquot in less than 100 μl.
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
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基因ID
- 6100
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UniProt
- P42212
抗原
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