His Tag 抗体 (HRP)
Our Local Distributor
北京 101111
Quick Overview for His Tag 抗体 (HRP) (ABIN401743)
抗原
See all His Tag 抗体宿主
克隆类型
标记
应用范围
-
-
序列
- HHHHHH
-
免疫原
- Synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide
-
-
anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB19960 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 3G5H8 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, ICC 宿主: 兔 Polyclonal HRP
anti-His Tag antibodyWB, IP 宿主: 兔 Monoclonal BLRE06M unconjugated Recombinant Antibody
-
-
应用备注
-
ELISA: 1/20,000. Western blot: 1/1,000 - 1/5,000. Immunohistochemistry on frozen sections: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
-
浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
-
缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA and 0.01 % (w/v) Gentamicin Sulfate
-
储存液
- Gentamicin sulfate
-
注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
-
注意事项
- This product is photosensitive and should be protected from light
-
储存条件
- 4 °C
-
储存方法
- Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE.
-
-
- His Tag
-
别名
- 6xHistidine Epitope Tag
-
物质类
- Tag
-
背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
抗原
-
