PRKDC 抗体 (Thr2609)
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Quick Overview for PRKDC 抗体 (Thr2609) (ABIN401263)
抗原
See all PRKDC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Thr2609
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特异性
- This antibody reacts with phosphorylated pThr2609 of DNA PKcs. Reactivity with non-phosphorylated Human DNA PKcs is minimal. Cross-reactivity with DNA PKcs homologues from other sources has not been determined.
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交叉反应 （详细）
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Species reactivity (expected):Chimpanzee (100 %), Mouse, Rat, Dog, Chicken.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
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免疫原
- Synthetic peptide corresponding to amino acids surrounding Thr 2609 of Human DNA PKcs
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亚型
- IgG
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anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 4019-4128) antibody
PRKDC 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibodyPRKDC 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 3H6 unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibodyKD Validated PRKDC 适用: 人 WB, IF (p), FACS 宿主: 兔 Monoclonal 23GB2055 unconjugated Recombinant Antibody
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibodyPRKDC 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (C-Term) antibodyPRKDC 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 151-300) antibodyPRKDC 适用: 人, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (C-Term) antibodyPRKDC 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 3899-4128) antibodyPRKDC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 4075-4104), (C-Term) antibodyPRKDC 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3465-3466 unconjugated
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (pThr2647) antibodyPRKDC 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: 1/300,000. Western blot: 1/500-1/2,000. Expect a band approx. 460 kDa in size corresponding to DNA PKcsin the appropriate cell lysate or extract. Best western blotting results are seen when Immunoprecipitation is performed prior todetection. This antibody detects an inducible signal at the correct height after DNAdamage. However, the antibody may also detect an IR-inducible signal in cells lacking DNAPKcs (MO59J cells) at the same size as DNA PKcs if western blotting is performed directly. We believe that this additional band is 53BP1 that runs at the same size as DNA PKcs and isalso phosphorylated in an IR-dependent manner. Similar results were seen with anotherphospho DNA PKcs antibody indicating that this result is general to antibodies to thisphospho site, rather than specific to this antibody.
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限制
- 仅限研究用
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浓度
- 0.93 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
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别名
- DNA-PKcs / PRKDC / XRCC7
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背景
- DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
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分子量
- 469.1 kDa
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基因ID
- 5591
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NCBI登录号
- NP_001075109
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UniProt
- P78527
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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