SUMO1 抗体
Quick Overview for SUMO1 抗体 (ABIN400792)
抗原
See all SUMO1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with yeast SUMO (Smt3).
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产品特性
- Synonyms: UBL1, GMP1, PIC1, Small ubiquitin-related modifier, Ubiquitin-like protein SMT3
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纯化方法
- Affinity chromatography on protein A
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免疫原
- Recombinant yeast SUMO protein
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亚型
- IgG1
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anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) antibody
SUMO1 适用: 人, 大鼠, 小鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) antibodySUMO1 适用: 人 WB, IF, IHC 宿主: 兔 Monoclonal unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (AA 1-101) antibodySUMO1 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3D7 unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) antibodySUMO1 适用: 人, 大鼠 WB, IF, IHC, FACS, StM 宿主: 小鼠 Monoclonal SM1-495 unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (N-Term) antibodySUMO1 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (AA 1-101) antibodySUMO1 适用: 人 WB, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (AA 1-30), (N-Term) antibodySUMO1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB0629 unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (AA 55-86) antibodySUMO1 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (AA 52-79) antibodySUMO1 适用: 人 WB, IF, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier Protein 1 (SUMO1) (AA 1-30) antibodySUMO1 适用: 人 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/20,000. Western blot: 1/2,000. Immunohistochemistry: 1/1,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.9 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody (in aliqouts) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))
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别名
- SMT3
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背景
- Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. However, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class, including parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkappaB-alpha, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa, and human SUMO-1 (a 101 amino acid polypeptide) shares 50 % sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18 % homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with beta-sheets wrapped around an alpha-helix.Synonyms: SMT-3, Ubiquitin-like protein SMT3
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基因ID
- 852122
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UniProt
- Q12306
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途径
- M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
抗原
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