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DDX58 抗体 (C-Term)

Name: DDX58 抗体 (C-Term)
SKU: ABIN388602
Availability: OutOfStock

This anti-DDX58 antibody is a 兔多克隆 antibody detecting DDX58 in WB. Suitable for 人和小鼠. This Primary Antibody has been cited in 9+ publications.

24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x106) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. (DDX58 抗体 (C-Term))

Western blot analysis of lysates from THP-1 cell line, untreated or treated with LPS, 1 μg/mL, using DDX58 C-term (upper) or Beta-actin (lower). (DDX58 抗体 (C-Term))

All lanes : Anti-DDX58 C-term at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: mouse spleen lysate Lane 5: mouse thymus lysate Lysates/proteins at 20 μg per lane. (DDX58 抗体 (C-Term))

3 images

(9 references)

产品编号 ABIN388602

发货至: 中国

Datasheet as PDF

Quick Overview for DDX58 抗体 (C-Term) (ABIN388602)

抗原

See all DDX58 抗体

DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This DDX58 antibody is un-conjugated

应用范围

Western Blotting (WB)

克隆位点

RB8008

Discover our top product DDX58 Primary Antibody

抗原表位
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AA 894-925, C-Term

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This RIG-I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 894-925 amino acids from the C-terminal region of human RIG-I.

亚型

Ig Fraction
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 550-776) antibody
DDX58 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 1-211) antibody
DDX58 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody
DDX58 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6C1 unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (C-Term) antibody
Verified DDX58 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 789-925) antibody
DDX58 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 4G1B6 unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (C-Term) antibody
DDX58 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 726-925) antibody
DDX58 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 200-250) antibody
DDX58 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (N-Term) antibody
DDX58 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 201-713) antibody
DDX58 适用: 人, 小鼠 WB, IHC, IP 宿主: 小鼠 Monoclonal Alme-1 unconjugated

应用备注

WB: 1:1000. WB: 1:2000. WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Munday, Howell, Barr, Hiscox: "Proteomic analysis of mitochondria in respiratory epithelial cells infected with human respiratory syncytial virus and functional implications for virus and cell biology." in: The Journal of pharmacy and pharmacology, Vol. 67, Issue 3, pp. 300-18, (2015) (PubMed).

Huang, Kolokoltsova, Yun, Seregin, Ronca, Koma, Paessler: "Highly Pathogenic New World and Old World Human Arenaviruses Induce Distinct Interferon Responses in Human Cells." in: Journal of virology, Vol. 89, Issue 14, pp. 7079-88, (2015) (PubMed).

Crill, Furr-Rogers, Marriott: "RIG-I is required for VSV-induced cytokine production by murine glia and acts in combination with DAI to initiate responses to HSV-1." in: Glia, (2015) (PubMed).

Feng, Langereis, Lork, Nguyen, Hato, Lanke, Emdad, Bhoopathi, Fisher, Lloyd, van Kuppeveld: "Enterovirus 2Apro targets MDA5 and MAVS in infected cells." in: Journal of virology, Vol. 88, Issue 6, pp. 3369-78, (2014) (PubMed).

Huang, Kolokoltsova, Yun, Seregin, Poussard, Walker, Brasier, Zhao, Tian, de la Torre, Paessler: "Junín virus infection activates the type I interferon pathway in a RIG-I-dependent manner." in: PLoS neglected tropical diseases, Vol. 6, Issue 5, pp. e1659, (2012) (PubMed).

Furr, Moerdyk-Schauwecker, Grdzelishvili, Marriott: "RIG-I mediates nonsegmented negative-sense RNA virus-induced inflammatory immune responses of primary human astrocytes." in: Glia, Vol. 58, Issue 13, pp. 1620-9, (2010) (PubMed).

Barral, Sarkar, Fisher, Racaniello: "RIG-I is cleaved during picornavirus infection." in: Virology, Vol. 391, Issue 2, pp. 171-6, (2009) (PubMed).

Bao, Liu, Shan, Li, Garofalo, Casola: "Human metapneumovirus glycoprotein G inhibits innate immune responses." in: PLoS pathogens, Vol. 4, Issue 5, pp. e1000077, (2008) (PubMed).

Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." in: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, (2008) (PubMed).
抗原

DDX58 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58))

别名

RIG-I

背景

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, RIG-I triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). This protein is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

分子量

106600

基因ID

23586

NCBI登录号

NP_055129

UniProt

O95786

途径

Activation of Innate immune Response, Hepatitis C