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ISG15 抗体 (N-Term)

Name: ISG15 抗体 (N-Term)
SKU: ABIN387983
Availability: OutOfStock

This anti-ISG15 antibody is a 兔多克隆 antibody detecting ISG15 in WB 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 5+ publications.

Western blot analysis of ISG15 (arrow) using rabbit polyclonal hISG15-A46 (ABIN387983 and ABIN2844984). (ISG15 抗体 (N-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (ISG15 抗体 (N-Term))

IHC (p)

ISG15 Antibody (A46) (ABIN387983 and ABIN2844984) western blot analysis in 293 cell line lysates (35 μg/lane). (ISG15 抗体 (N-Term))

ISG15 Antibody western blot analysis in Jurkat? (ISG15 抗体 (N-Term))

4 images

(5 references)

产品编号 ABIN387983

发货至: 中国

Quick Overview for ISG15 抗体 (N-Term) (ABIN387983)

抗原

See all ISG15 抗体

ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

适用

人

宿主

兔

克隆类型

多克隆

标记

This ISG15 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB02791

Discover our top product ISG15 Primary Antibody

抗原表位
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AA 31-61, N-Term

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human ISG15.

亚型

Ig Fraction
anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) antibody
ISG15 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) antibody
ISG15 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (Middle Region) antibody
ISG15 适用: 人, 兔, Cow, 犬, 马, Pig, 绵羊, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 31-61) antibody
ISG15 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) antibody
ISG15 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 39-68), (N-Term) antibody
ISG15 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) antibody
ISG15 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 80-129) antibody
ISG15 适用: 人, 兔, Cow, 犬, 马, Pig, 绵羊 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (C-Term) antibody
ISG15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157) antibody
ISG15 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Cheriyamundath, Basu, Haase, Doernberg, Gavert, Brabletz, Ben-Zeev: "ISG15 induction is required during L1-mediated colon cancer progression and metastasis." in: Oncotarget, Vol. 10, Issue 67, pp. 7122-7131, (2019) (PubMed).

Speer, Li, Buta, Payelle-Brogard, Qian, Vigant, Rubino, Gardner, Wedeking, Hermann, Duehr, Sanal, Tezcan, Mansouri, Tabarsi, Mansouri, Francois-Newton, Daussy, Rodriguez, Lenschow, Freiberg et al.: "ISG15 deficiency and increased viral resistance in humans but not mice. ..." in: Nature communications, Vol. 7, pp. 11496, (2018) (PubMed).

Katzenell, Leib: "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." in: Journal of virology, (2016) (PubMed).

Huang, Bulavin: "Oncogene-mediated regulation of p53 ISGylation and functions." in: Oncotarget, Vol. 5, Issue 14, pp. 5808-18, (2015) (PubMed).

Schanz, Baston-Büst, Heiss, Beyer, Krüssel, Hess: "Interferon stimulated gene 15 expression at the human embryo-maternal interface." in: Archives of gynecology and obstetrics, Vol. 290, Issue 4, pp. 783-9, (2014) (PubMed).
抗原

ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

别名

ISG15

背景

ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.

分子量

17888

基因ID

9636

NCBI登录号

NP_005092

UniProt

P05161