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His Tag 抗体 (FITC)

Name: His Tag 抗体 (FITC)
SKU: ABIN387722
Availability: OutOfStock

This anti-His Tag antibody is a 小鼠单克隆 antibody detecting His Tag in WB 和 ELISA. Suitable for . This Primary Antibody has been cited in 5+ publications.

1 image

(5 references)

产品编号 ABIN387722

发货至: 中国

Quick Overview for His Tag 抗体 (FITC) (ABIN387722)

抗原

See all His Tag 抗体

His Tag

适用

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宿主

小鼠

克隆类型

单克隆

标记

This His Tag antibody is conjugated to FITC

应用范围

Western Blotting (WB), ELISA

克隆位点

6G2A9

Discover our top product His Tag Primary Antibody

品牌

THE™

序列

HHHHHH

特异性

THE^TM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

产品特性

This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

纯化方法

Protein A affinity column

免疫原

A synthetic peptide HHHHHH coupled - KLH

亚型

IgG
anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 800)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800

anti-His Tag antibody (DyLight 649)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

anti-His Tag antibody (HRP)
WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/mL
Flow cytometry: 0.1-2 µg for 1 x 10^6 cells
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

浓度

1 mg/mL

缓冲液

PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freezing and thawing cycles.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Yang, Wahlmüller, Sarg, Furtmüller, Geiger: "A+-helix of protein C inhibitor (PCI) is a cell-penetrating peptide that mediates cell membrane permeation of PCI." in: The Journal of biological chemistry, Vol. 290, Issue 5, pp. 3081-91, (2015) (PubMed).

Jarmander, Janoschek, Lundh, Larsson, Gustavsson: "Process optimization for increased yield of surface-expressed protein in Escherichia coli." in: Bioprocess and biosystems engineering, Vol. 37, Issue 8, pp. 1685-93, (2014) (PubMed).

An, Budd, Kanost, Michel: "Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes." in: Cellular and molecular life sciences : CMLS, Vol. 68, Issue 11, pp. 1929-39, (2011) (PubMed).

Oliveira, Lindblad: "Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 15, pp. 3804-14, (2011) (PubMed).

Li, Wang, Zhou, Yang, Bhaskar, Hu, Sun, Miao: "Cloning and expression of a cellulase gene in the silkworm, Bombyx mori by improved Bac-to-Bac/BmNPV baculovirus expression system." in: Molecular biology reports, Vol. 37, Issue 8, pp. 3721-8, (2010) (PubMed).
抗原

His Tag

物质类

Tag