beta Actin 抗体 (HRP)
Quick Overview for beta Actin 抗体 (HRP) (ABIN7477941)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Anti-beta-Actin [HRP] Monoclonal Antibody (Mouse) reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig substrates. It has not yet been tested in other species.
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交叉反应 (详细)
- Cross reactivity in other species has not yet been tested.
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产品特性
- This product provides a useful and serves as a specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Do not add azide because it inhibits the activity of HRP.
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纯化方法
- Protein G affinity column
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免疫原
- A synthetic peptide DDDIAALVVDNGSG coupled - KLH
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亚型
- IgG
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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: "Optimization of oncogene expression through intra-population competition." in: Biotechnology journal, Vol. 8, Issue 12, pp. 1476-84, (2013) (PubMed).
: "Dynamics of the Cag-type IV secretion system of Helicobacter pylori as studied by bacterial co-infections." in: Cellular microbiology, Vol. 15, Issue 11, pp. 1924-37, (2013) (PubMed).
: "Oncogenic features of the JMJD2A histone demethylase in breast cancer." in: International journal of oncology, Vol. 41, Issue 5, pp. 1701-6, (2012) (PubMed).
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: "Optimization of oncogene expression through intra-population competition." in: Biotechnology journal, Vol. 8, Issue 12, pp. 1476-84, (2013) (PubMed).
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- beta Actin
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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