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His Tag 抗体 (Biotin)

WB, ELISA 宿主: 小鼠 Monoclonal 6G2A9 Biotin
产品编号 ABIN387702
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This His Tag antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), ELISA
    品牌
    THE™
    序列
    HHHHHH
    特异性
    THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
    产品特性
    The antibody is suitable for detecting fusion proteins that contain a His-tag and it is supplied as a 100 µL aliquot at a concentration of 1 mg/mL.
    纯化方法
    Protein A affinity column
    免疫原
    A synthetic peptide (HHHHHH) coupled - KLH
    克隆位点
    6G2A9
    亚型
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.02-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    0.5 mg/mL
    缓冲液
    PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freezing and thawing cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Wegman, Geuze, van der Helm, Cumhur Öner, Dhert, Alblas: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." in: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).

    Ota, Doyle-Cooper, Cooper, Huber, Falkowska, Doores, Hangartner, Le, Sok, Jardine, Lifson, Wu, Mascola, Poignard, Binley, Chakrabarti, Schief, Wyatt, Burton, Nemazee: "Anti-HIV B Cell lines as candidate vaccine biosensors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 10, pp. 4816-24, (2012) (PubMed).

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