His Tag 抗体 (Biotin)
(2 references)Quick Overview for His Tag 抗体 (Biotin) (ABIN387702)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- THE™
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序列
- HHHHHH
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特异性
- THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
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产品特性
- The antibody is suitable for detecting fusion proteins that contain a His-tag and it is supplied as a 100 µL aliquot at a concentration of 1 mg/mL.
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纯化方法
- Protein A affinity column
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免疫原
- A synthetic peptide (HHHHHH) coupled - KLH
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亚型
- IgG
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
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应用备注
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Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- 0.5 mg/mL
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缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." in: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).
: "Anti-HIV B Cell lines as candidate vaccine biosensors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 10, pp. 4816-24, (2012) (PubMed).
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: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." in: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).
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- His Tag
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物质类
- Tag
抗原
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