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His Tag 抗体 (HRP)

Name: His Tag 抗体 (HRP)
SKU: ABIN387701
Availability: OutOfStock

This anti-His Tag antibody is a 小鼠单克隆 antibody detecting His Tag in WB 和 ELISA. Suitable for . This Primary Antibody has been cited in 6+ publications.

Lane 1: MarkerLane 2: 10 ng Multiple Tag Cell Lysate fusion protein (ABIN1536505) Lane 3: 1 ng Multiple Tag Cell Lysate fusion protein (ABIN1536505) Lane 4: Negative controlDetect antibody: 1 µg/mL Anti-His [HRP] Monoclonal Antibody (ABIN387701) (His Tag 抗体 (HRP))

1 image

(6 references)

产品编号 ABIN387701

发货至: 中国

Quick Overview for His Tag 抗体 (HRP) (ABIN387701)

抗原

See all His Tag 抗体

His Tag

适用

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宿主

小鼠

克隆类型

单克隆

标记

This His Tag antibody is conjugated to HRP

应用范围

Western Blotting (WB), ELISA

克隆位点

6G2A9

Discover our top product His Tag Primary Antibody

品牌

THE™

序列

HHHHHH

特异性

THE^TM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

产品特性

This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.

纯化方法

Protein A affinity column

免疫原

A synthetic peptide HHHHHH coupled - KLH

亚型

IgG
anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 800)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800

anti-His Tag antibody (DyLight 649)
WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

anti-His Tag antibody (HRP)
WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

浓度

0.5 mg/mL

缓冲液

PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing cycles.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, (2015) (PubMed).

Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." in: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, (2015) (PubMed).

Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." in: PLoS ONE, Vol. 9, Issue 9, pp. e107683, (2014) (PubMed).

Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." in: Cancer research, Vol. 74, Issue 24, pp. 7510-20, (2014) (PubMed).

Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." in: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, (2013) (PubMed).

Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." in: Microbial cell factories, Vol. 11, pp. 118, (2012) (PubMed).
抗原

His Tag

物质类

Tag