CD25 抗体 (SPRD)
Quick Overview for CD25 抗体 (SPRD) (ABIN375573)
抗原
See all CD25 (IL2RA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- Spectral Red ™
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特异性
- Mouse CD25, Mr 55 kDa
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产品特性
- Rat Anti-Mouse CD25-SPRD
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纯化方法
- Purified
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免疫原
- IL-2 dependent BALB/c helper T cell clone HT2
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亚型
- IgG2b
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应用备注
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Neut - Reported in literature
- Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY7, and AF700 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
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说明
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Inhibition of IL-2 or mitogen-induced T cell proliferation, In vitro blocking of IL-2 binding
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样本量
- 1 mL
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- CD25
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背景
- The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. It is expressed at a higher level on CD4 + CD8 + thymocytes. It is also expressed on cultured epidermal Langerhans cells. The biochemical features of murine CD25 have been characterized in detail.
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原
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