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CD25 抗体 (PE-Cy7)

This 大鼠 单克隆 antibody specifically detects CD25 in FACS. It exhibits reactivity toward 小鼠.
产品编号 ABIN375559
发货至: 中国
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Quick Overview for CD25 抗体 (PE-Cy7) (ABIN375559)

抗原

See all CD25 (IL2RA) 抗体
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

适用

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小鼠

宿主

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大鼠

克隆类型

  • 308
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单克隆

标记

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This CD25 antibody is conjugated to PE-Cy7

应用范围

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Flow Cytometry (FACS)

克隆位点

3C7
  • 特异性

    Mouse CD25, Mr 55 kDa

    产品特性

    Rat Anti-Mouse CD25-PE/CY7

    纯化方法

    Purified

    免疫原

    IL-2 dependent BALB/c helper T cell clone HT2

    亚型

    IgG2b
  • 应用备注

    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Neut - Reported in literature
    • Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY7, and AF700 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

    说明

    Inhibition of IL-2 or mitogen-induced T cell proliferation, In vitro blocking of IL-2 binding

    样本量

    1 mL

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    0.1 mg in 0.2 mL of PBS/Sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C
  • 抗原

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    别名

    CD25

    背景

    The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. It is expressed at a higher level on CD4 + CD8 + thymocytes. It is also expressed on cultured epidermal Langerhans cells. The biochemical features of murine CD25 have been characterized in detail.

    途径

    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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