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GAPDH 抗体 (Internal Region)

Name: GAPDH 抗体 (Internal Region)
SKU: ABIN374640
Availability: OutOfStock

GAPDH 适用: 人, 小鼠, 大鼠, 犬 WB, IHC (fro), EIA 宿主: 山羊 Polyclonal unconjugated

Image no. 1 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody (ABIN374640) (GAPDH 抗体 (Internal Region))

1 image

(4 references)

产品编号 ABIN374640

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
37

21

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
212

142

135

75

65

55

36

28

23

21

20

19

18

16

12

12

11

10

9

8

6

4

4

4

4

3

3

3

3

3

2

2

1

1

1

1

1
人, 小鼠, 大鼠, 犬
宿主
159

97

17

10

4
山羊
Compatible Secondaries
克隆类型
180

105
多克隆
标记
156

35

22

16

5

5

5

5

4

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
234

98

73

70

46

41

35

30

16

16

15

15

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

序列

C-GVNHEKYDNS LK

特异性

This antibody is specific to GAPDH (Internal). GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.

交叉反应（详细）

Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat.

纯化方法

Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

免疫原

Peptide with sequence from the internal region of the protein sequence according to NP_002037.2. Genename: GAPDH

Top Product

Discover our top product GAPDH Primary Antibody
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Peptide ELISA: 1/4000 (Antibody detection limit dilution). Western Blot: 0.1-0.3 μg/mL. A approx. 36 kDa band is observed in Human Liver, Testes and Tonsillysates and in Mouse Spleen lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, (2009) (PubMed).

Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).

Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).

Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH (GAPDH 产品)

别名

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

背景

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

分子量

36.1kDa.

基因ID

2597

NCBI登录号

NP_002037

UniProt

P04406