14-3-3 sigma/SFN 抗体 (Internal Region, N-Term)
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- 抗原 See all 14-3-3 sigma/SFN (SFN) 抗体
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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抗原表位
- Internal Region, N-Term
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适用
- 人, 小鼠, 大鼠, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This 14-3-3 sigma/SFN antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 序列
- C-DLHTLSEDSY KDST
- 特异性
- This antibody recognizes 4-3-3 sigma/Stratifin.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Canine (Dog) and Porcine.
Species reactivity (tested):Human. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_006133.1.
- Top Product
- Discover our top product SFN Primary Antibody
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anti-Stratifin (SFN) antibody
SFN 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (Internal Region) antibodyVerified SFN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Stratifin (SFN) (C-Term) antibodySFN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 140-156) antibodySFN 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) antibodySFN 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-SFN-2 unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Stratifin (SFN) (AA 1-249) antibodySFN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (AA 140-156), (Middle Region) antibodySFN 适用: 人 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: 1/64000. Western Blot: 0.1-0.3 μg/mL. Approx.28 kDa band observed in Human Skin lysates (calculated MW of 27.8 kDa accordingto NP_006133.1). Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Skin, this antibody shows strong nuclear staining in selectedcells of the basal layer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- 别名
- 14-3-3 Protein sigma / SFN (SFN 产品)
- 别名
- YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody
- 背景
- Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.Synonyms: Epithelial cell marker protein 1, HME1, Stratifin
- 基因ID
- 2810, 9606
- UniProt
- P31947
- 途径
- p53 Pathway, Myometrial Relaxation and Contraction
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