GAPDH 抗体 (C-Term)
(10 references)-
- 抗原 See all GAPDH 抗体
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠, 犬
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GAPDH antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- C-HQVVSSDFNS DT
- 特异性
- This antibody is specific to GAPDH at C-Term. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.
- 交叉反应 （详细）
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Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide with sequence from the C-Terminus of the protein sequence according to NP_002037.2.
- Top Product
- Discover our top product GAPDH Primary Antibody
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH 适用: 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Peptide ELISA: Detection Limit: 1/2000Western Blot: 0.01-0.03 μg/mL. A approx. 35 kDa band is observed in lysates of Human Tonsil,MouseSpleen, and cell lines HeLa, A431 and 293. Immunohistochemistry In Paraffin Embedded Tonsil: 0.3 μg/mL. Staining of cells ingerminal centre, mantle zone, interfollicular area, epithelium and endothelium is
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial-Mesenchymal Transition: Effects on Anoikis." in: Molecular cancer research : MCR, Vol. 14, Issue 6, pp. 528-38, (2017) (PubMed).
: "Pseudosyndactyly - an inflammatory and fibrotic wound healing disorder in recessive dystrophic epidermolysis bullosa." in: Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG, Vol. 13, Issue 12, pp. 1257-66, (2016) (PubMed).
: "Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways." in: BMC research notes, Vol. 8, pp. 785, (2016) (PubMed).
: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." in: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, (2014) (PubMed).
: "Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 1, pp. G126-35, (2010) (PubMed).
: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." in: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, (2008) (PubMed).
: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." in: PLoS ONE, Vol. 3, Issue 2, pp. e1631, (2008) (PubMed).
: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, (2008) (PubMed).
: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." in: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, (2007) (PubMed).
: "Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome." in: Histochemistry and cell biology, Vol. 126, Issue 2, pp. 251-9, (2006) (PubMed).
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: "Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial-Mesenchymal Transition: Effects on Anoikis." in: Molecular cancer research : MCR, Vol. 14, Issue 6, pp. 528-38, (2017) (PubMed).
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- 抗原
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- 别名
- GAPDH (GAPDH 产品)
- 别名
- G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
- 背景
- Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
- 分子量
- 36.1 kDa (Calculated)
- 基因ID
- 2597
- NCBI登录号
- NP_002037
- UniProt
- P04406
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