NOXO1 抗体 (C-Term)
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Quick Overview for NOXO1 抗体 (C-Term) (ABIN374411)
抗原
See all NOXO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- RGCVDSVPHP TTEQ
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特异性
- This antibody detects NOXO1 at C-term. It is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).
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交叉反应 （详细）
- Species reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_653204.1, NP_751907.1, NP_751907.1. Genename: NOXO1
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anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 304-376) antibody
NOXO1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyVerified NOXO1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 1-100) antibodyNOXO1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyNOXO1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 121-370) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (C-Term) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 192-219) antibodyNOXO1 适用: 人 WB 宿主: 兔 Polyclonal RB37554 unconjugated
anti-NADPH Oxidase Organizer 1 (NOXO1) (AA 238-252) antibodyNOXO1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 16000. Immunohistochemistry: 10-20 μg/mL. In paraffin embedded Human Kidney Cortex showscytoplasmic stain in epithelial cells of some PCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))
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别名
- NOXO1
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背景
- NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Cotransfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5 P2, PtdIns 5 P and PtdIns 4 P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 colocalizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5
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基因ID
- 124056
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NCBI登录号
- NP_653204
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UniProt
- Q8NFA2
抗原
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