TCP1 alpha/CCTA 抗体 (C-Term)
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Quick Overview for TCP1 alpha/CCTA 抗体 (C-Term) (ABIN374406)
抗原
See all TCP1 alpha/CCTA (TCP1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-SYEDAVHSGA LND
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特异性
- This antibody detects CCT1 / TCP1 at C-term. It is expected to recognise both reported isoforms.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1. Genename: TCP1
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anti-T-Complex 1 (TCP1) (AA 1-556) antibody
TCP1 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2B2-D6 unconjugated
anti-T-Complex 1 (TCP1) (C-Term) antibodyVerified TCP1 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (AA 82-98) antibodyTCP1 适用: 人, 小鼠, 大鼠 WB, IF, FACS, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Complex 1 (TCP1) (AA 515-551), (C-Term) antibodyTCP1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-T-Complex 1 (TCP1) (C-Term) antibody (HRP)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a HRP
anti-T-Complex 1 (TCP1) (C-Term) antibody (FITC)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a FITC
anti-T-Complex 1 (TCP1) (C-Term) antibody (Biotin)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a Biotin
anti-T-Complex 1 (TCP1) (C-Term) antibody (Atto 390)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a Atto 390
anti-T-Complex 1 (TCP1) (C-Term) antibody (Atto 594)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a Atto 594
anti-T-Complex 1 (TCP1) (C-Term) antibody (Atto 488)TCP1 适用: 小鼠 WB, IF, FACS, IP, ICC 宿主: 大鼠 Monoclonal 91a Atto 488
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应用备注
- Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60 kDa band observed in Hela, Human Lung and HumanTestis lysates (predicted MW of 60 kDa according to NP_110379.2). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Testis shows onlycytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining inother germ cells with unstained nucleoli.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
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别名
- CCT1 / TCP1
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背景
- TCP1 alpha is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.Synonyms: CCT alpha, CCTA, Chaperonin containing T complex polypeptide 1 subunit 1, T-complex protein 1 subunit alpha, TCP-1-alpha, TCP1 alpha
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基因ID
- 6950
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NCBI登录号
- NP_110379
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UniProt
- P17987
抗原
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