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TCP1 alpha/CCTA 抗体 (C-Term)

Name: TCP1 alpha/CCTA 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN185185
Availability: OutOfStock

Verified TCP1 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 山羊 Polyclonal unconjugated

ABIN185185 staining (1 ug/ml) of Human Lung lysate (RIPA buffer, 35 ug total protein per lane). (TCP1 alpha/CCTA 抗体 (C-Term))

ABIN185185 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (TCP1 alpha/CCTA 抗体 (C-Term))

FACS

ABIN185185 (10µg/ml) staining of paraffin embedded Human Testis. (TCP1 alpha/CCTA 抗体 (C-Term))

IHC

ABIN185185 (1µg/ml) staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35µg protein in RIPA buffer). (TCP1 alpha/CCTA 抗体 (C-Term))

ABIN185185 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (TCP1 alpha/CCTA 抗体 (C-Term))

5 images

产品编号 ABIN185185

发货至: 中国

Datasheet as PDF

抗原 See all TCP1 alpha/CCTA (TCP1) 抗体

TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
抗原表位
46

18

17

15

15

15

9

5

4

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
146

110

58

23

21

20

18

18

17

16

13

11

10

4

4

3

2

2

2

1

1
人
宿主
127

36

19

1
山羊
Compatible Secondaries
克隆类型
119

64
多克隆
标记
66

13

11

10

5

5

5

5

5

5

4

4

4

4

4

4

3

3

3

3

3

2

2

2

2

2

2

1

1
This TCP1 alpha/CCTA antibody is un-conjugated
应用范围
134

48

48

44

42

40

39

31

25

20

11

10

3

2

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

原理

TCP1

序列

SYEDAVHSGA LND

特异性

This antibody is expected to recognise both reported isoforms.

交叉反应

人

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

质量等级

Verified

免疫原

Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1.

亚型

IgG

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TCP1 适用: 人 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated

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TCP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

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TCP1 适用: 小鼠 WB, IF, IP, FACS, ICC 宿主: 大鼠 Monoclonal 91a unconjugated

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TCP1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-063R unconjugated Recombinant Antibody

anti-T-Complex 1 (TCP1) (C-Term) antibody (HRP)
TCP1 适用: 小鼠 WB, IF, IP, FACS, ICC 宿主: 大鼠 Monoclonal 91a HRP

anti-T-Complex 1 (TCP1) (C-Term) antibody (FITC)
TCP1 适用: 小鼠 WB, IF, IP, FACS, ICC 宿主: 大鼠 Monoclonal 91a FITC

应用备注

Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2 μg/mL.
Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3 kDa according to NP_110379.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.

说明

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

别名

TCP1 (TCP1 产品)

别名

CCT-alpha antibody, CCT1 antibody, CCTa antibody, D6S230E antibody, TCP-1-alpha antibody, AI528772 antibody, CCT antibody, Cct1 antibody, Ccta antibody, TRic antibody, Tcp-1 antibody, Tp63 antibody, c-cpn antibody, p63 antibody, TRiC antibody, CCTalpha antibody, BEST:GH05123 antibody, CG5374 antibody, Dmel\\CG5374 antibody, T-cpl antibody, TCP-1alpha antibody, TCPA_DROME antibody, Tcp1 antibody, Tcp1-alpha antibody, gh05123 antibody, cct-alpha antibody, ccta antibody, tcp1 antibody, tcp1-a antibody, tcp1a antibody, tcp1alpha antibody, CHUNP6875 antibody, fa13h08 antibody, wu:fa13h08 antibody, wu:fc95g06 antibody, t-complex 1 antibody, T-complex protein 1 subunit alpha antibody, t-complex protein 1 antibody, Tcp1-like antibody, t-complex 1 S homeolog antibody, TCP1 antibody, cct-1 antibody, Tcp1 antibody, T-cp1 antibody, tcp1.S antibody, tcp1 antibody

背景

TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1

基因ID

6950

NCBI登录号

NP_110379, NP_001008897