TRIM5 抗体 (N-Term)
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Quick Overview for TRIM5 抗体 (N-Term) (ABIN374320)
抗原
See all TRIM5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- ASGILVNVKE EVTC
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特异性
- This antibody detects TRIM5 / RNF88 at N-term. It is expected to recognise all three human isoforms of this protein.
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交叉反应 (详细)
- Species reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1. Genename: TRIM5
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应用备注
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 37 kDa band observed in Human Brain lysates (calculatedMW of 37.7 kDa according to NP_149084.1, isoform delta). An additional faint 47 kDa bandconsistent with calculated MW of 40.7 kDa according to NP_149083.1 (isoform gamma) isalso observed. Immunohistochemistry: 1.0 μg/mL. In paraffin embedded Human Tonsil,stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptaeand weak in germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- TRIM5 (Tripartite Motif Containing 5 (TRIM5))
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别名
- TRIM5 / RNF88
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背景
- Synonyms: RING finger protein 88, Tripartite motif-containing protein 5
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基因ID
- 85363
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NCBI登录号
- NP_149023
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UniProt
- Q9C035
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途径
- Activation of Innate immune Response
抗原
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