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TRIM5 抗体 (N-Term)

This 山羊 多克隆 antibody specifically detects TRIM5 in WB 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN374320
发货至: 中国
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Quick Overview for TRIM5 抗体 (N-Term) (ABIN374320)

抗原

See all TRIM5 抗体
TRIM5 (Tripartite Motif Containing 5 (TRIM5))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This TRIM5 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    N-Term

    序列

    ASGILVNVKE EVTC

    特异性

    This antibody detects TRIM5 / RNF88 at N-term. It is expected to recognise all three human isoforms of this protein.

    交叉反应 (详细)

    Species reactivity (tested):Human

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1. Genename: TRIM5
  • 应用备注

    Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 37 kDa band observed in Human Brain lysates (calculatedMW of 37.7 kDa according to NP_149084.1, isoform delta). An additional faint 47 kDa bandconsistent with calculated MW of 40.7 kDa according to NP_149083.1 (isoform gamma) isalso observed. Immunohistochemistry: 1.0 μg/mL. In paraffin embedded Human Tonsil,stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptaeand weak in germinal centre.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • 抗原

    TRIM5 (Tripartite Motif Containing 5 (TRIM5))

    别名

    TRIM5 / RNF88

    背景

    Synonyms: RING finger protein 88, Tripartite motif-containing protein 5

    基因ID

    85363

    NCBI登录号

    NP_149023

    UniProt

    Q9C035

    途径

    Activation of Innate immune Response
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