BIRC7 抗体 (C-Term)
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Quick Overview for BIRC7 抗体 (C-Term) (ABIN374091)
抗原
See all BIRC7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-RAPVRSRVRT
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特异性
- This antibody is expected to recognise both reported isoforms of BIRC7 (as represented by NP_647478.1 and NP_071444.1)
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_647478.1, NP_071444.1. Genename: BIRC7
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应用备注
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Peptide ELISA: Limit dilution 1: 4000. Western blot: 0.1 - 1.0 μg/mL. Experiments gave bands at approx 30 kDa and 30 kDa inhuman acute lymphoblastic leukaemia MOLT4 lysate after 0.3 μg/mL antibody staining. These bands correspond to the reported isoforms with calculated MW of 32.0 kDaaccording to NP_647478.1 and 30.0 kDa according to NP_071444.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
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别名
- BIRC7 / LIVIN
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背景
- Livin is a novel member of the IAP protein family and contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin alpha and Livin beta. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. The two isoforms of Livin appear to have different functions and tissue distributions.Synonyms: Baculoviral IAP repeat-containing protein 7, KIAP, Kidney inhibitor of apoptosis protein, Livin, MLIAP, Melanoma inhibitor of apoptosis protein, RING finger protein 50, RNF50
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基因ID
- 79444
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NCBI登录号
- NP_647478
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UniProt
- Q96CA5
抗原
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