NCF1 抗体 (C-Term)
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Quick Overview for NCF1 抗体 (C-Term) (ABIN374079)
抗原
See all NCF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-SESTKRKLAS AV
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特异性
- This antibody detects NCF-1 at C-term.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat, Rabbit, Pig, Cow.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from the C Terminus of the protein according to NP_000256.3. Genename: NCF1
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应用备注
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Peptide ELISA: Limit dilution 1: 20000. Western blot: 0.2-2 μg/mL. Approx 47 kDa band seen in U937 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
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别名
- NCF1
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背景
- NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD).Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NOXO2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, SH3PXD1A, p47-phox
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基因ID
- 653361
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NCBI登录号
- NP_000256
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UniProt
- P14598
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途径
- PI3K-Akt Signaling
抗原
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