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NCF1 抗体 (C-Term)

Verified NCF1 适用: 人, 小鼠, Pig WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184583
发货至: 中国
  • 抗原 See all NCF1 抗体
    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
    抗原表位
    • 28
    • 16
    • 11
    • 10
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 115
    • 41
    • 37
    • 16
    • 7
    • 3
    • 2
    • 2
    • 2
    • 2
    人, 小鼠, Pig
    宿主
    • 128
    • 1
    • 1
    山羊
    克隆类型
    • 128
    • 1
    多克隆
    标记
    • 66
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This NCF1 antibody is un-conjugated
    应用范围
    • 97
    • 63
    • 55
    • 26
    • 22
    • 14
    • 14
    • 13
    • 7
    • 6
    • 4
    • 4
    • 3
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    NCF1 / p47phox
    序列
    SESTKRKLAS AV
    交叉反应
    Cow, 人, 小鼠, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
    亚型
    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45

    Peptide ELISA: antibody detection limit dilution 1:20000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
    别名
    NCF1 (NCF1 产品)
    别名
    NCF1A antibody, NOXO2 antibody, SH3PXD1A antibody, p47phox antibody, Ncf-1 antibody, p47 antibody, neutrophil cytosolic factor 1 antibody, NCF1 antibody, Ncf1 antibody
    背景
    NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
    基因ID
    653361, 17969, 114553
    NCBI登录号
    NP_000256
    途径
    PI3K-Akt Signaling
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