SYN1 抗体 (pSer9)
Quick Overview for SYN1 抗体 (pSer9) (ABIN372718)
抗原
See all SYN1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer9
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特异性
- This antibody recognizes the ~78k Synapsin I doublet protein phosphorylated at Ser9. The antibody also weakly labels the ~55k Synapsin II protein which has a similar phosphorylation site to that of Ser9 on Synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda-phosphatase.
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交叉反应 （详细）
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Species reactivity (expected):Human, Bovine, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat. -
纯化方法
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
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免疫原
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of Synapsin I.
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亚型
- IgG
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anti-Synapsin I (SYN1) (AA 362-511) antibody
SYN1 适用: 人, 大鼠, 小鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7H10G6 unconjugated
anti-Synapsin I (SYN1) (AA 113-420) antibodySYN1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) (AA 362-450) antibodySYN1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H1 unconjugated
anti-Synapsin I (SYN1) (pSer9) antibodySYN1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) (pSer9) antibodySYN1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) (pSer9) antibodySYN1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) antibodySYN1 适用: 人, 大鼠, 小鼠, Cow WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) (pSer62), (pSer67) antibodySYN1 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) (pSer549) antibodySYN1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Synapsin I (SYN1) (AA 201-300) antibodySYN1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1/1,000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at-20 °C.
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- SYN1 (Synapsin I (SYN1))
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别名
- Synapsin-1
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背景
- Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989, Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).Synonyms: Brain protein 4.1, SYN1, Synapsin I
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基因ID
- 6853
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NCBI登录号
- NP_008881
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UniProt
- P17600
抗原
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