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- 抗原 See all ERK1/2 (MAPK1/3) 抗体
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ERK1/2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- This antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively.
- 产品特性
- Synonyms: MAPK1, ERK2, PRKM1, PRKM2, ERK-2, ERT1, ERT2, PRKM3, MAPK3, ERK-1, ERK1,Mitogen-activated protein kinase, Extracellular signal-regulated kinase, Insulin-stimulatedMAP2 kinase
- 纯化方法
- Purified
- 免疫原
- Human recombinant Erk1 and Erk2.
- 克隆位点
- SB46b
- 亚型
- IgG2a
- Top Product
- Discover our top product MAPK1/3 Primary Antibody
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anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 301-358) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibodyMAPK1/3 适用: 人, 小鼠, 大鼠, Cow, Pig, 斑马鱼, 犬, 绵羊, 猴, 马, 鱼 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibodyMAPK1/3 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr183), (pTyr185) antibodyMAPK1/3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr185), (pThr202) antibodyMAPK1/3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (C-Term) antibodyMAPK1/3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibodyMAPK1/3 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3F8 unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr183), (pTyr185) antibodyMAPK1/3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (phosphospecific) antibodyMAPK1/3 适用: 人, 小鼠, 大鼠 WB, ELISA, IP 宿主: 兔 Monoclonal G15-B unconjugated
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibodyMAPK1/3 适用: 人, 小鼠 WB, ELISA, DB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1-2 μg/mL. Predicted Mol. Weight: 42 and 44 kDa. Immunoprecipitation: 10-15 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.05 mg/mL
- 缓冲液
- 100 mM Borate buffered saline, pH 8.0 without preservatives.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- 别名
- ERK1 / ERK2 (MAPK1/3 产品)
- 背景
- Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases. (Ref.1-4) Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85 % identical and have higher identity in the core regions involved in substrate binding. (Ref.4) Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. (Ref.4,5) Both sites must be phosphorylated for maximum activity. (Ref.1-4) These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus. (Ref.1-3)Synonyms: ERK-1/ERK-2, Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAPK1/MAPK2, Mitogen-activated protein kinase, P42/P44-MAPK
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