The antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 13.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pTyr13 (Q-Q-Y (p) -E-C) derived from Human CDK6. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.