AKT 1/2/3 抗体 (pTyr312, pTyr315, pTyr316)
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Quick Overview for AKT 1/2/3 抗体 (pTyr312, pTyr315, pTyr316) (ABIN362434)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr312, pTyr315, pTyr316
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特异性
- The antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pTyr315, 316, 312 (P-E-Y (p) -L-A) derived from Human AKT1, AKT2, AKT3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-AKT 1/2/3 (AA 401-479) antibody
适用: 人, 小鼠, 大鼠, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer473) antibody适用: 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 鱼 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer472) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 antibody适用: 人, 小鼠, 大鼠, Chimpanzee WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 14E5-16C8-25F6 unconjugated
anti-AKT 1/2/3 (pTyr474) antibody适用: 人, 小鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (AA 278-312) antibody适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB43841 unconjugated
anti-AKT 1/2/3 (AA 431-480) antibody适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- AKT 1/2/3
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别名
- AKT1/AKT2/AKT3
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背景
- General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
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分子量
- 60 kDa
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NCBI登录号
- NP_005456, NP_001617
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UniProt
- Q9Y243
抗原
-
