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SMAD2 抗体 (pThr220)

This anti-SMAD2 antibody is a 兔 多克隆 antibody detecting SMAD2 in WB. Suitable for 人, 小鼠 和 大鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN362418
发货至: 中国

Quick Overview for SMAD2 抗体 (pThr220) (ABIN362418)

抗原

See all SMAD2 抗体
SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This SMAD2 antibody is un-conjugated

应用范围

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Western Blotting (WB)
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    pThr220

    特异性

    The antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pThr220 (P-E-T (p) -P-P) derived from Human Smad2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Zhang, Shen, Zhang, Wan, Yao, Wu, Wang, Chen, Yan, Jiang: "Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice." in: PLoS ONE, Vol. 8, Issue 3, pp. e59002, (2013) (PubMed).

  • 抗原

    SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

    别名

    Smad2

    背景

    Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

    分子量

    60 kDa

    UniProt

    Q15796

    途径

    Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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