The antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
免疫原
Peptide sequence around phosphorylation site of pThr187 (E-Q-T (p) -P-K) derived from Human p27Kip1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.