The antibody detects endogenous level of p21Cip1 only when phosphorylated at threonine 145.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pThr145 (R-Q-T (p) -S-M) derived from Human p21Cip1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.