14-3-3 zeta 抗体 (pSer58)
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Quick Overview for 14-3-3 zeta 抗体 (pSer58) (ABIN362197)
抗原
See all 14-3-3 zeta (YWHAZ) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer58
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特异性
- The antibody detects endogenous level of 14-3-3 z only when phosphorylated at serine 58.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer58 (R-S-S (p) -W-R) derived from Human 14-3-3 zeta. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-14-3-3 zeta (YWHAZ) (AA 133-212) antibody
YWHAZ 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 19G7E9 unconjugated
anti-14-3-3 zeta (YWHAZ) (Center) antibodyYWHAZ 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 51-150) antibodyYWHAZ 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B3 unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 133-212) antibodyYWHAZ 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (pSer58) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (Tyr330) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 200-245) antibodyYWHAZ 适用: 人, 小鼠, 大鼠, Cow, 犬, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 1-100) antibodyYWHAZ 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (pThr232) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (C-Term) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- 14-3-3 zeta (YWHAZ)
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别名
- 14-3-3 zeta
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背景
- This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
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分子量
- 28 kDa
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NCBI登录号
- NP_001129
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UniProt
- P63104
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途径
- Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
抗原
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