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HSP27 抗体 (pSer15)

Name: HSP27 抗体 (pSer15)
SKU: ABIN362172
Availability: OutOfStock

HSPB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

(1 reference)

产品编号 ABIN362172

发货至: 中国

Datasheet as PDF

抗原 See all HSP27 (HSPB1) 抗体

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
抗原表位
41

36

27

16

16

10

8

8

7

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer15
适用
297

104

90

24

14

14

13

12

11

6

6

4

3

2

2

2

1
人
宿主
235

66
兔
Compatible Secondaries
克隆类型
207

93
多克隆
标记
135

23

17

13

12

8

7

7

7

7

7

7

4

4

4

4

4

4

4

3

3

3

3

3

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
应用范围
254

104

104

95

69

50

49

46

41

40

16

12

10

7

7

2

2

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

特异性

The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

纯化方法

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

免疫原

Peptide sequence around phosphorylation site of pSer15 (G-P-S (p) -W-D) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

亚型

IgG

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anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
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anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
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应用备注

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Park, Kudo, Hagiwara, Tabuchi, Watanabe, Munakata, Sakurai: "p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression." in: Free radical biology & medicine, Vol. 52, Issue 11-12, pp. 2284-91, (2012) (PubMed).
抗原

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

别名

HSP27 (HSPB1 产品)

别名

CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

背景

Involved in stress resistance and actin organization.

分子量

27 kDa

NCBI登录号

NP_001531

UniProt

P04792

途径

MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling