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HSP27 抗体 (pSer15)

HSPB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN362171
发货至: 中国
  • 抗原 See all HSP27 (HSPB1) 抗体
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    抗原表位
    • 40
    • 33
    • 32
    • 16
    • 16
    • 8
    • 8
    • 6
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    pSer15
    适用
    • 295
    • 99
    • 86
    • 19
    • 13
    • 13
    • 13
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
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    宿主
    • 234
    • 66
    克隆类型
    • 208
    • 91
    多克隆
    标记
    • 133
    • 20
    • 15
    • 11
    • 10
    • 7
    • 5
    • 5
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    • 5
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    This HSP27 antibody is un-conjugated
    应用范围
    • 245
    • 102
    • 99
    • 87
    • 63
    • 47
    • 46
    • 42
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    • 40
    • 15
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    特异性
    The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.
    纯化方法
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
    免疫原
    Peptide sequence around phosphorylation site of pSer15 (G-P-S (p) -W-D) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    亚型
    IgG
    Top Product
    Discover our top product HSPB1 Primary Antibody
  • 应用备注
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Park, Kudo, Hagiwara, Tabuchi, Watanabe, Munakata, Sakurai: "p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression." in: Free radical biology & medicine, Vol. 52, Issue 11-12, pp. 2284-91, (2012) (PubMed).

  • 抗原
    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
    别名
    HSP27 (HSPB1 产品)
    背景
    Involved in stress resistance and actin organization.
    分子量
    27 kDa
    NCBI登录号
    NP_001531
    UniProt
    P04792
    途径
    MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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