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HSP27 抗体 (pSer15)

This anti-HSP27 antibody is a 兔 多克隆 antibody detecting HSP27 in WB, IHC 和 IF. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN362172
发货至: 中国

Quick Overview for HSP27 抗体 (pSer15) (ABIN362172)

抗原

See all HSP27 (HSPB1) 抗体
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

适用

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宿主

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克隆类型

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多克隆

标记

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This HSP27 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
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    pSer15

    特异性

    The antibody detects endogenous level of HSP27only when phosphorylated at serine 15.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

    免疫原

    Peptide sequence around phosphorylation site of pSer15 (G-P-S (p) -W-D) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    亚型

    IgG
  • 应用备注

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Park, Kudo, Hagiwara, Tabuchi, Watanabe, Munakata, Sakurai: "p38MAPK suppresses chronic pancreatitis by regulating HSP27 and BAD expression." in: Free radical biology & medicine, Vol. 52, Issue 11-12, pp. 2284-91, (2012) (PubMed).

  • 抗原

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    别名

    HSP27

    背景

    Involved in stress resistance and actin organization.

    分子量

    27 kDa

    NCBI登录号

    NP_001531

    UniProt

    P04792

    途径

    MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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