CDC25A 抗体 (pSer76)
(1 reference)Quick Overview for CDC25A 抗体 (pSer76) (ABIN362128)
抗原
See all CDC25A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer76
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特异性
- The antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pSer76 (M-G-S (p) -S-E) derived from Human cdc25A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 235-484) antibody
CDC25A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibodyCDC25A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07634 unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer75) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibodyCDC25A 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 1-524) antibodyCDC25A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer178) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (N-Term) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (Ser124) antibodyCDC25A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB59086 unconjugated
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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: "CK1ε targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 3, pp. 531-7, (2011) (PubMed).
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: "CK1ε targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 3, pp. 531-7, (2011) (PubMed).
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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别名
- cdc25A
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背景
- Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
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分子量
- 70 kDa
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NCBI登录号
- NP_001780
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UniProt
- P30304
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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