CDK2 抗体 (pThr160)
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Quick Overview for CDK2 抗体 (pThr160) (ABIN362119)
抗原
See all CDK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr160
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特异性
- The antibody detects endogenous level of CDK2 only when phosphorylated at threonine 160.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around phosphorylation site of pThr160 (T-Y-T (p) -H-E) derived from Human CDK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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亚型
- IgG
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应用备注
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
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别名
- CDK2
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背景
- Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
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分子量
- 34 kDa
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NCBI登录号
- NP_001789
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UniProt
- P24941
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原
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