Estrogen Receptor alpha 抗体 (pSer167)
Our Local Distributor
北京 101111
Quick Overview for Estrogen Receptor alpha 抗体 (pSer167) (ABIN362012)
抗原
See all Estrogen Receptor alpha (ESR1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer167
-
特异性
- The antibody detects endogenous level of Estrogen Receptor-a only when phosphorylated at serine16.
-
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
-
免疫原
- Peptide sequence around phosphorylation site of pSer167 (L-A-S (p) -T-N) derived from Human Estrogen Receptor-a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
-
亚型
- IgG
-
-
-
-
应用备注
-
Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
-
-
: "Expression of estrogen receptor beta and phosphorylation of estrogen receptor alpha serine 167 correlate with progression-free survival in patients with metastatic breast cancer treated with ..." in: Oncology, Vol. 79, Issue 1-2, pp. 55-61, (2011) (PubMed).
-
-
- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
-
别名
- Estrogen Receptor-alpha
-
背景
- Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
-
分子量
- 66 kDa
-
NCBI登录号
- NP_000116
-
UniProt
- P03372
-
途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
-