LAMP2 抗体
Quick Overview for LAMP2 抗体 (ABIN361746)
抗原
See all LAMP2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ~100-110 kDa.
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交叉反应
- 人, 小鼠, 兔
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纯化方法
- Protein G Purified
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免疫原
- Purified preparation of mouse liver lysosomal membranes
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亚型
- IgG1
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anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 191-362) antibody
LAMP2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 29-168) antibodyLAMP2 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 6A10H10 unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 29-168) antibodyLAMP2 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 8E2F2 unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 301-411) antibodyLAMP2 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 193-375) antibodyLAMP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibodyLAMP2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibodyLAMP2 适用: 人 WB, FACS, ICC, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal H4B4 unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibodyKO Validated LAMP2 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 54-268) antibodyLAMP2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (AA 30-127) antibodyLAMP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G10 unconjugated
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应用备注
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- WB (1:1000)
- ICC/IF (1:500)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN361745 was sufficient for detection of LAMP2 in 20 μg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- -20°C
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- LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))
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别名
- LAMP2
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背景
- Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C4 and prostaglandin D2, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).
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基因ID
- 16784
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NCBI登录号
- NP_001017959
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UniProt
- P17047
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途径
- Autophagy
抗原
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