MYLK 抗体 (N-Term)
Quick Overview for MYLK 抗体 (N-Term) (ABIN360383)
抗原
See all MYLK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody reacts to MLCK.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MLCK-long.
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亚型
- Ig Fraction
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anti-Myosin Light Chain Kinase (MYLK) (AA 1375-1524) antibody
MYLK 适用: 人, 小鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7F2B11 unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1684-1884) antibodyMYLK 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1441-1709) antibodyMYLK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1765-1914) antibodyMYLK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 923-953), (N-Term) antibodyMYLK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB03067 unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 908-938) antibodyMYLK 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1661-1877) antibodyMYLK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1-992) antibodyMYLK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (C-Term) antibodyMYLK 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 290-398) antibodyMYLK 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MYLK (Myosin Light Chain Kinase (MYLK))
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别名
- MYLK
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背景
- MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.Synonyms: Kinase-related protein, MLCK, Myosin light chain kinase, Telokin, smooth muscle
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基因ID
- 4638, 5874
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UniProt
- Q15746
抗原
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