ARAF 抗体 (Middle Region)
Quick Overview for ARAF 抗体 (Middle Region) (ABIN360127)
抗原
See all ARAF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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特异性
- This antibody reacts to A-RAF.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human ARAF1.
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亚型
- Ig Fraction
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anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 151-250) antibody
ARAF 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibodyARAF 适用: 人, 小鼠 WB, IF (cc), IHC (p) 宿主: 兔 Monoclonal 24D1 unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) antibodyARAF 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibodyARAF 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 160-310) antibodyARAF 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr301), (pTyr302) antibodyARAF 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pSer299) antibodyARAF 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibodyARAF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 276-325), (pTyr302) antibodyARAF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibodyARAF 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
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别名
- ARAF
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背景
- Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
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基因ID
- 369, 9606
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UniProt
- P10398
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途径
- MAPK Pathway, RTK signaling, Hepatitis C
抗原
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