MKNK2 抗体 (N-Term)
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北京 101111
Quick Overview for MKNK2 抗体 (N-Term) (ABIN359132)
抗原
See all MKNK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody reacts to MNK2.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MKNK2.
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亚型
- Ig Fraction
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anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-158) antibody
MKNK2 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2A10 unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-158) antibodyMKNK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4F11 unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 36-85) antibodyMKNK2 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 斑马鱼, Bat, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 41-158) antibodyMKNK2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (N-Term) antibodyMKNK2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 402-432), (C-Term) antibodyMKNK2 适用: 人 WB 宿主: 兔 Polyclonal RB5513 unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-100) antibodyMKNK2 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-414) antibodyMKNK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (AA 1-158) antibodyMKNK2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2) (Internal Region) antibodyMKNK2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))
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别名
- MKNK2
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背景
- MAP kinase-interacting kinase 1 (Mnk1) and Mnk2, members of the Ser/Thr protein kinase family, bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2. Erk and p38 phosphorylate MNK1 and Mnk2, which stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E). Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. This ubiquitiously expressed protein appears to regulate transcription by phosphorylating eIF-4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94 % identical) and human Mnk1 (71 % identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta. Mnk2 has also been shown to interact with the C-terminal regions of eIF-4G1 and eIF-4G2.Synonyms: GPRK7, MAP kinase-interacting serine/threonine-protein kinase 2, MNK2
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基因ID
- 2872, 9606
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UniProt
- Q9HBH9
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途径
- MAPK Pathway
抗原
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