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PKC alpha 抗体 (C-Term)

Name: PKC alpha 抗体 (C-Term)
SKU: ABIN359058
Availability: OutOfStock

PKCa 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

Western blot analysis of anti-PKCalpha Pab in placenta lysate. (PKC alpha 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (PKC alpha 抗体 (C-Term))

IHC (p)

2 images

产品编号 ABIN359058

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
24

20

18

12

11

10

8

7

7

6

6

5

5

5

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
178

90

68

6

5

5

3

2

2

1

1

1

1
人
宿主
174

12

1
兔
Compatible Secondaries
克隆类型
140

46
多克隆
标记
96

15

13

6

6

6

6

6

6

6

6

6

2

2

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
128

71

53

33

22

20

17

15

14

8

5

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

特异性

This antibody reacts to PKC alpha.

纯化方法

Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

免疫原

This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.

亚型

Ig Fraction

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anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IP, IF, ICC 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠 WB, FACS, IHC (p), IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
PKCa 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody
PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
状态

Liquid

浓度

0.25 mg/mL

缓冲液

PBS with 0.09 % (W/V) sodium azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PRKCA (PKCa 产品)

别名

pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

背景

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC alpha is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type

基因ID

5578, 9606

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling