This antibody detects Cdc25A pSer79. Predicted to cross react with Mouse (100 % Antigen Homology).
纯化方法
Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
免疫原
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S79 of human Cdc25A.
ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原
CDC25A
(Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
别名
CDC25A
背景
CDC25A is a member of the CDC25 family of phosphatases.CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division.CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1