CDC25A 抗体 (pSer76)
Quick Overview for CDC25A 抗体 (pSer76) (ABIN358097)
抗原
See all CDC25A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer76
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特异性
- This antibody detects CDC25A pSer75.
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S75 of human Cdc25A.
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亚型
- Ig Fraction
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anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 235-484) antibody
CDC25A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibodyCDC25A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07634 unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer75) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibodyCDC25A 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 1-524) antibodyCDC25A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer178) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (N-Term) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (Ser124) antibodyCDC25A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB59086 unconjugated
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应用备注
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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别名
- CDC25A
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背景
- CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
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分子量
- 59087 Da
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基因ID
- 993, 9606
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UniProt
- P30304
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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