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BID 抗体 (pSer65)

This anti-BID antibody is a 兔 多克隆 antibody detecting BID in WB, IHC (p) 和 EIA. Suitable for 人.
产品编号 ABIN358089
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for BID 抗体 (pSer65) (ABIN358089)

抗原

See all BID 抗体
BID (BH3 Interacting Domain Death Agonist (BID))

适用

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宿主

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克隆类型

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多克隆

标记

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This BID antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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    pSer65

    特异性

    This antibody detects BID pSer65.

    纯化方法

    Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.

    亚型

    Ig Fraction
  • 应用备注

    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.25 mg/mL

    缓冲液

    PBS with 0.09 % (W/V) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    BID (BH3 Interacting Domain Death Agonist (BID))

    别名

    BID

    背景

    Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID

    分子量

    26836 Da

    基因ID

    637

    NCBI登录号

    NP_001187

    UniProt

    P55957

    途径

    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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