PPT1 抗体 (C-Term)
Quick Overview for PPT1 抗体 (C-Term) (ABIN357784)
抗原
See all PPT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody detects PPT1 (C-term).
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纯化方法
- Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PPT1.
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亚型
- Ig Fraction
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anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 100-306) antibody
PPT1 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 1-306) antibodyPPT1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1117CT11-2-1-4 unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) antibodyPPT1 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G4 unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 1-306) antibodyPPT1 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 100-306) antibodyPPT1 适用: 人, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 188-290) antibodyPPT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 28-279) antibodyPPT1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 100-258) antibodyPPT1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 191-224), (C-Term) antibodyPPT1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Palmitoyl-Protein Thioesterase 1 (PPT1) (AA 269-300), (C-Term) antibodyPPT1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4707 unconjugated
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应用备注
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ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))
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别名
- PPT1 / PPT
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背景
- Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18 % amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle, variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.Synonyms: PPT-1, Palmitoyl-protein hydrolase 1, Palmitoyl-protein thioesterase 1
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分子量
- 34193 Da
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基因ID
- 5538, 5874
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UniProt
- P50897
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途径
- SARS-CoV-2 Protein Interactome
抗原
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