SMURF1 抗体 (N-Term)
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Quick Overview for SMURF1 抗体 (N-Term) (ABIN357469)
抗原
See all SMURF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody detects SMURF1 at N-term.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SMURF1.
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亚型
- Ig Fraction
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anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) antibody
SMURF1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 198-374) antibodySMURF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibodySMURF1 适用: 人, 小鼠 WB, IF, IHC (p), EIA, IP 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96), (N-Term) antibodySMURF1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB04101 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96) antibodySMURF1 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-269) antibodySMURF1 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 1-731) antibodySMURF1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 711-740), (C-Term) antibodySMURF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4103 unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) antibodySMURF1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (C-Term) antibodySMURF1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
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别名
- SMURF1
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背景
- Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.Synonyms: E3 ubiquitin-protein ligase SMURF1, KIAA1625, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF 1, SMURF-1
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分子量
- 86113 Da
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基因ID
- 57154, 9606
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UniProt
- Q9HCE7
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途径
- Regulation of Cell Size
抗原
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